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  1. #1
    Join Date
    Jan 2010
    Location
    Alachua County, FL, USA
    Posts
    7,050

    Default BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses

    BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses
    Most of the currently characterized honeybee viruses (around twenty) are positive stranded RNA viruses. Techniques based on RNA signatures are widely used to determine the viral load in honeybee colonies. A new multiparameter assay, called “BeeDoctor”, was developed based on multiplex-ligation probe dependent amplification (MLPA) technology. This assay detects 10 honeybee viruses in one reaction. The “BeeDoctor” assay was used to screen 363 apparently healthy colonies from randomly selected apiaries throughout Flanders. This survey showed that almost 80% of colonies are infected with at least one virus, and many with multiple infections, showing that virus infections in apiaries are quite common, even in the absence of clinical symptoms.

    http://www.plosone.org/article/info%...l.pone.0047953
    americasbeekeeper.com
    beekeeper@americasbeekeeper.com

  2. #2
    Join Date
    Aug 2005
    Location
    Washington County, Maine
    Posts
    2,960

    Default Re: BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses

    Thank you for posting this. I've looked at the PLOSONE report, didn't understand most of what the report said, and was left with a question:

    How is the BeeDoctor technique going to make me a better beekeeper?
    Master Beekeeper (EAS) and Master Gardener (U Maine CE) www.beeberrywoods.com

  3. #3
    Join Date
    Feb 2001
    Location
    Enfield,Ct.
    Posts
    470

    Default Re: BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses

    " virus infections in apiaries are quite common,"

    I'm not sure this is a bad thing.Since the only "cure " for a virus is the host's immune system,wouldn't this at low levels impart some immunity for a healthy host.(I'm thinking flu shot)

  4. #4
    Join Date
    Oct 2011
    Location
    Santa Monica, CA, USA
    Posts
    1,532

    Default Re: BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses

    In many cases, virus itself does not kill. Secondary bacterial (or other problem) infection kills. I do not think, that this test is anyhow useful from practical point of view. If somebody developed the test for human - they will discover that we are full of horrible viruses, but ... alive! In normal situation, immune system keeps viruses under control.
    Серёжа, Sergey

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