Results 1 to 3 of 3

Hybrid View

  1. #1
    Join Date
    Jan 2010
    Location
    Alachua County, FL, USA
    Posts
    6,435

    Default You are what you eat - siRNAs may be transferred to the pollinators

    Host Generated siRNAs Attenuate Expression of Serine Protease Gene in Myzus persicae
    Beneficial pollinators viz. Pieris rapae and Mamestra configurata occur on members of the family Brassicaceae while Apis mellifera on Asteraceae members along-with M. persicae. It is presumed that siRNAs may be transferred to the pollinators when they suck the plant sap. To examine any homology between the targeted MySP sequence to its orthologous counterparts in beneficial pollinators, serine protease gene sequences of pollinator insects Apis mellifera, (Accession no. NM_001011584), Tribolium castaneum (Accession no. NM_001170771), Mamestra configurata (Accession no. FJ205442), and Pieris rapae (Accession no. FJ882067) from GenBank were aligned using Clustal W program for generating a phylogenetic tree (Fig. 2).
    http://www.plosone.org/article/info%...l.pone.0046343
    americasbeekeeper.com
    beekeeper@americasbeekeeper.com

  2. #2
    Join Date
    Feb 2010
    Location
    New York City, NY
    Posts
    4,030

    Default Re: You are what you eat - siRNAs may be transferred to the pollinators

    "In Cluster 2, M. persicae and A. mellifera evolved from a common ancestor but diverged 56.05% between them."

    So, they've tested transgenic Arabidopsis, expressing a host specific siRNA, against a serine protease, in the green peach aphid.

    The siRNA shouldn't affect Honeybees.

    "The MySP transcripts showed variable but significant reductions in the nymphs (inoculated and parthenogenetic progenies) across all the developmental stages when fed on SP-transgenic lines."

    It seems to work.

  3. #3
    Join Date
    Jan 2010
    Location
    Alachua County, FL, USA
    Posts
    6,435

    Default Re: You are what you eat - siRNAs may be transferred to the pollinators

    The point to beekeepers is not that siRNA affect bees, but it is the delivery method for all current RNAi research an production.
    americasbeekeeper.com
    beekeeper@americasbeekeeper.com

Tags for this Thread

Bookmarks

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Ads