9/30/2012.

http://www.biomedcentral.com/1471-2164/9/301

Differential gene expression of the honey bee Apis mellifera associated with Varroa destructor infection
M Navajas1*, A Migeon1†, C Alaux2†, ML Martin-Magniette3,4, GE Robinson2, JD Evans5, S Cros-Arteil1, D Crauser6 and Y Le Conte6 s

Please read: We measured differences in gene expression in two colonies of Varroa-susceptible and two colonies of Varroa-tolerant bees.

Background
The parasitic mite, Varroa destructor, is the most serious pest of the western honey bee, Apis mellifera, and has caused the death of millions of colonies worldwide. This mite reproduces in brood cells and parasitizes immature and adult bees. We investigated whether Varroa infestation induces changes in Apis mellifera gene expression, and whether there are genotypic differences that affect gene expression relevant to the bee's tolerance, as first steps toward unravelling mechanisms of host response and differences in susceptibility to Varroa parasitism.
Results
We explored the transcriptional response to mite parasitism in two genetic stocks of A. mellifera which differ in susceptibility to Varroa, comparing parasitized and non-parasitized full-sister pupae from both stocks. Bee expression profiles were analyzed using microarrays derived from honey bee ESTs whose annotation has recently been enhanced by results from the honey bee genome sequence. We measured differences in gene expression in two colonies of Varroa-susceptible and two colonies of Varroa-tolerant bees. We identified a set of 148 genes with significantly different patterns of expression: 32 varied with the presence of Varroa, 116 varied with bee genotype, and 2 with both. Varroa parasitism caused changes in the expression of genes related to embryonic development, cell metabolism and immunity
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