Re: first treatment with OA
I have been pondering this issue of application of OA in order to insure that all cells are treated while uncapped. This requires a careful look at both the capped cycle of drone and worker brood cells.
Drone cells are capped for 14 days. worker cells are capped for 13 days.
1 second before you apply OA a drone cell is capped and the treatment is in effective. In addition one second after the treatment is in effective a drone begins to emerge that the treatment also missed. I am saying the treatment was effective for 10 minutes.
Assuming that the queen lays a new egg immediately that freshly opened cell will be capped again in 11 days for a drone, 8 days for a worker cell. To insure that all cells that have been opened since the last application you must retreat in 7 days or less. Still half the cells that where capped at first treatment are still capped at second treatment. to unsure those cells are treated while open at third treatment you must treat at 14 to 15 days from the first application.
THis might help it make more since.
Drone brood has 14 days in which it is capped. Numbers cells 1 through 14. Worker cells are capped for 13 days numbers cells 1 thru 13 application one treats all open cells and no capped cells for either worker cells or drone cells.
During day 7 all drone cells 8 thru 14 have emerged and are still open cells. also all worker cells 8 thru 13 have emerged and are still open.
Note the cell that had a bee emerge one second after first application was no longer effective will be recapped on day 9. waiting ten days to treat will result in this cell being capped for all three applications. You only have 24 hours to treat this cell when uncapped.
During day 14 from fist treatment all remaining cells that where originally capped will be open. IN this case you have a window of as much as 72 hours before they will be capped again.
I have seen it recommended that you treat with AO three times spaced 10 days apart. to as frequently as three to four times 7 days apart. as the above close look indicates. 10 days apart will fail to treat all cells. 7 days is a tight fit for the first two applications with a bit more wiggle room for the third application. 10 days does not work for any of the three applications. cells will be missed at every treatment.
Due to this I suggest 3 treatments spaced strictly, 7 days apart.
Significant Minority vs Insignificant Majority, 20% of causes produce 80% of the effects.