Results 1 to 20 of 32

Hybrid View

  1. #1
    Join Date
    Jan 2005
    Location
    Southern Oregon
    Posts
    1,162

    Default Hygenic test results on breeders



    Last edited by JBJ; 06-16-2012 at 01:56 PM. Reason: adding pics and testing size
    John B Jacob www.oldsolbees.com

  2. #2
    Join Date
    Jan 2005
    Location
    Southern Oregon
    Posts
    1,162

    Default Re: Hygenic test results on breeders

    These are just a few of the great results at the 24 hour mark. We arrived at a very hygienic bee by selecting for Varroa tolerance. Dr Royce from OSU came down and showed us how to to the freeze killed brood assay. There were some that did not get 100% in the test so I presume they must have other mechanisms for coping with mites. I have been leaning towards the ones that got 100% for the last few grafts but will continue to monitor the other breeders for mite loads and overall success.
    John B Jacob www.oldsolbees.com

  3. #3
    Join Date
    Jan 2005
    Location
    Southern Oregon
    Posts
    1,162

    Default Re: Hygenic test results on breeders

    The one labeled 13.1 actually cleaned both sides of the comb. I think we may have used too much liquid N and froze it all the way through.
    John B Jacob www.oldsolbees.com

  4. #4
    Join Date
    May 2012
    Location
    Cheyenne, Wyoming, USA
    Posts
    1,693

    Default Re: Hygenic test results on breeders

    That amazing! I gotta try that method.

  5. #5
    Join Date
    Apr 2012
    Location
    Baytown, TX., USA.
    Posts
    651

    Default Re: Hygenic test results on breeders

    Good work! Another person that did this for years was S. Taber, don't see much mention of his work anymore.

  6. #6
    Join Date
    Feb 2011
    Location
    Meadows of Dan, Virginia, United States
    Posts
    195

    Default Re: Hygenic test results on breeders

    Where do you get your nitrogen, and what do you use to store it?

  7. #7
    Join Date
    Jan 2005
    Location
    Southern Oregon
    Posts
    1,162

    Default Re: Hygenic test results on breeders

    We used a loaner dewar from a local welding supply shop. I think it was about 10 or 12 liters.

  8. #8
    Join Date
    Mar 2012
    Location
    Calhoun Co, Texas, USA
    Posts
    1,310

    Default Re: Hygenic test results on breeders

    is there any reason why "dry ice" wouldn't work for this purpose? I know it's plenty easier to come by for the average hobbyist/sideliner beek...hopefully it'd work well too?

  9. #9
    Join Date
    Dec 2005
    Location
    Brasher Falls, NY, USA
    Posts
    26,812

    Default Re: Hygenic test results on breeders

    What were the mite loads like? Does this indicate varroa mite tolerance or resistance? I can see how it would indicate hygenic behavior, but, does hygenic behavior directly lead to mite tolerance/resistance?

    What other traits are you selecting for and how do you go about accessing the degree of those traits?
    Mark Berninghausen "That which works, persists."

  10. #10
    Join Date
    Mar 2012
    Location
    Orange county, Texas
    Posts
    175

    Default Re: Hygenic test results on breeders

    How did you get them to lay around that circle?
    I am a nebee so i am just learning about the bees

  11. #11
    Join Date
    Dec 2005
    Location
    Brasher Falls, NY, USA
    Posts
    26,812

    Default Re: Hygenic test results on breeders

    The OPer can answer from experience, but, you start off w/ a nice patch of capped brood and kill what you can see as an empty circle of brood by applying liquid nitrogen, which freezes the pupae dead. Exactly how this is done, someone else needs to explain. I think a tin can is used somehow, but I haven't done it.
    Mark Berninghausen "That which works, persists."

  12. #12
    Join Date
    Jan 2005
    Location
    souris, manitoba, canada
    Posts
    758

    Default Re: Hygenic test results on breeders

    what is the relationship between a cell that has been killed by liquid nitrogen and a cell that has a foundress mite mite in it

  13. #13
    Join Date
    Jan 2005
    Location
    Southern Oregon
    Posts
    1,162

    Default Re: Hygenic test results on breeders

    Quote Originally Posted by sqkcrk View Post
    What were the mite loads like? Does this indicate varroa mite tolerance or resistance? I can see how it would indicate hygenic behavior, but, does hygenic behavior directly lead to mite tolerance/resistance?

    What other traits are you selecting for and how do you go about accessing the degree of those traits?
    We are looking for hardy mite tolerant bees that are productive. We will be doing some sugar rolls soon to put some metrics on current mite loads. All breeders wintered well and easily made the grade for the almonds. We, of course, are looking for all of the usual economically valuable traits. I like a bee that can pay the bills.
    Varroa sensitive hygiene is a well documented trait and does result in lower mite loads. Apparently you can end up with very hygienic bees by selecting from bees that cope with mites well. It is a challenge to keep the selection pressure on but well worth doing. We are taking some steps this year to try to get these traits more "true breeding" throughout the whole population. More isolated mating yards and instrumental insemination will definitely be helpful this year.
    John B Jacob www.oldsolbees.com

  14. #14
    Join Date
    Dec 2005
    Location
    Brasher Falls, NY, USA
    Posts
    26,812

    Default Re: Hygenic test results on breeders

    Thanks. I can tell you've really thought about this. What are your plans? Queens for yourself or for selling to others?
    Mark Berninghausen "That which works, persists."

Bookmarks

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Ads