Beesource Beekeeping Forums banner

Harvard study on neonics and bee deaths - download full text

20K views 48 replies 18 participants last post by  deknow 
#1 ·
Read about it in THE HARVARD GAZETTE HERE:

http://news.harvard.edu/gazette/story/2012/04/pesticide-tied-to-bee-colony-collapse/

We now have the full text of the Harvard Study by Alex Lu which can be downloaded from this Google Docs link:


https://docs.google.com/open?id=0B7FCgF0BwlDGZXZVWFFqUUtRTGl5Uzdyb21VRV9Bdw

DISCUSSION ABSTRACT:


Discussion

The magnitude and the pattern of honey bee hive loss
during the winter months in this study resemble the reported
symptoms of CCD. The loss of 15 of 16 imidacloprid-
treated hives (94%) across 4 apiaries occurred
over a period of 10 weeks following the first hive death.
Dead hives were remarkably empty except for stores of
food and some pollen left on the frames (figure 3). The
dead hives, particularly for those treated with higher dosages
of imidacloprid, was preceded by the observation
of dead bees scattered on snow in front of the hives,
with diminished small clusters remaining the week before
death. Snow usually fell between weekly hive examinations
making the observation of scattered dead
honey bees in front of individual hives noticeable. Although
this observation is not quite reminiscent of the
reported CCD symptoms, it is important to consider that
if these hives were located in a warmer climate region,
such as in Florida USA where migratory hives overwinter,
bees exiting the hives would have dispersed some
distance from the hives and therefore would not be observed
in front of the hives.

The replicated controlled design of this in situ study in
the apiarian setting, and the survival of honey bees in 3
of 4 control hives (figure 4), eliminate the possibility
that hive deaths were caused by common suggested risk
factors, such as long-distance transportation of hives,
malnutrition, or the reported toxic effect of hydroxymethylfurfural,
a heat-formed contaminant during the
distillation process of making HFCS, to honey bees (Le-
Blanc et al., 2009). We used the same HFCS in both the
imidacloprid-treated and control hives. The loss of imidacloprid-
treated hives in this study is also highly unlikely
due to pathogen infection since the presence of neither
Nosema nor a large number of Varroa mites was
observed in hives during the summer and fall seasons.
In addition, all hives were treated with Apistan strips
and Fumagillin B, two effective treatments for parasite
prevention, prior to the winter season. Since all hives
were considered healthy as they went into fall season,
those pathogens posed very little threat to the health of
honey bee hives. The only dead control hive exhibited
symptoms of dysentery in which dead honey bees were
found both inside and outside of the hive, which is not
seen in the other 19 hives.


Data from this in situ study provide convincing evidence
that exposure to sub-lethal levels of imidacloprid
causes honey bees to exhibit symptoms consistent to
CCD months after imidacloprid exposure.
Should stressor
factors other than feeding honey bees with HFCS
containing imidacloprid cause CCD, the loss of honey
bees would not occur disproportionally on those imidacloprid-
treated hives. The survival of the control hives
unequivocally augments this conclusion. The study hypothesis
is further supported by the mortality data presented
in figure 2, which clearly demonstrates a dose-
response relationship, in which the highest imidacloprid
dose exterminates hives more quickly than the subsequent
doses in all 4 apiaries. Although imidacloprid, and
other neonicotinoid insecticides have been suggested as
a possible contributing factor to CCD because of its toxicity
in impairing foraging ability or triggering other
neuro-behavioral problems (e.g. failure to return to the
hive) in honey bees at sub-lethal doses (Suchail et al.,
2001; Rortais et al., 2005; Thompson and Maus, 2007;
Yang et al., 2008; Mullin et al., 2010), its attribution to
CCD in the apiary setting has never been documented.
The results from this study underscore the paucity of
research concerning the sub-lethal effects of pesticides
on CCD, particularly of neonicotinoids throughout the
yearly life cycle of entire honey bee colonies under
natural conditions (Maini et al., 2010; Spivak et al.,
2011).
 
See less See more
#5 ·
If you read the study and not the press hype, all it proved is that if you feed bees insecticide they die. Nothing new or novel about that finding and certainly not the cause of CCD. Not every CCD hive was fed HFCS. Further, none of my hives collapsed this year and I had dead bees in the snow. It's a terrible study. Amazing that Harvard would have approved it.
 
#7 ·
Re: Imidacoprid linked to CCD

Forget calling it CCD - that's like "natural causes" it just means that they died and we don't know why. Except systemic pesticides making thousands of acres of forage toxic to all insects in general (it's intended purpose) is NOT natural causes. Neonics are almost certainly one of the major causes of hive collapse. If you were TRYING to kill bees you could hardly design a better tool for it. Even if it's not THE cause of "CCD" it's still a big problem - assuming we want to continue having bees.
 
#9 ·
Re: Imidacoprid linked to CCD

The results were hardly shocking.
They fed bees enough insecticide to kill them...the 20ppb dose (the smallest dose they used) is the same dose the study claims whole kill bees....and according to the studies they cited, 0.1ppb should kill bees if fed over 10 days.
the symptoms they caused are not consistant with CCD....in fact, they didn't reference a single definition of CCD...just a superficial description.
no one has ever documented any imidacloprid in HFCS...they cite one.person claiming to have found unquantifiable levels....but no data (and the Harvard team claims.they can quantify down to 0.5ppb).
if you do watch the video, please note the explanation of bt corn....and if it makes sense, please explain it to me.

Deknow
 
#14 · (Edited)
Re: Imidacoprid linked to CCD

The results were hardly shocking.
They fed bees enough insecticide to kill them...the 20ppb dose (the smallest dose they used) is the same dose the study claims whole kill bees....and according to the studies they cited, 0.1ppb should kill bees if fed over 10 days.
the symptoms they caused are not consistant with CCD....in fact, they didn't reference a single definition of CCD...just a superficial description.
no one has ever documented any imidacloprid in HFCS...they cite one.person claiming to have found unquantifiable levels....but no data (and the Harvard team claims.they can quantify down to 0.5ppb).
if you do watch the video, please note the explanation of bt corn....and if it makes sense, please explain it to me.

Deknow
The significance of this study lies in the fact that the imidacoprid didn't kill the bees until 3 months after the last treatment!

If you read the study carefully then you will have to admit that they didn't try to kill the bees, they wanted to show what long term effect very small doses have on the colony, and the effect was this:

Of the treated hives a staggering 94 % died of CCD like symptoms while only one of the 4 controls died, but of different symptoms (dysentery).


Don't you think we should start folow-up studies immediately to verify the effect, or are you scared of such experiments?
 
#11 ·
I cant reference the exact year, if it was the 80's then it was the early 80's. They called it disappearing disease. I remember having bought some queens from Parks that year and getting a call later in the summer from them asking if I had noticed any disappearing disease. I thought he was joking at first but then he related a lot of experiences some folks out in California were experiencing. Of course this was the good old days of beekeeping when you could easily get devastated with massive losses from foliar spraying. Of course there was the federal indemnity program that reimbursed you if you got your claim in early enough before the funds ran dry.
 
#12 ·
And Isle of Wight Disease before that. But, same thing.

My "winterloss" was 12% this year. Two friends report 5% and 7% each. These are beekeepers w/ more than 500 and the other 1,000 colonies. Is this an indication of a lessening of the problem? Probably just anecdotal evidence. It would be nice to think it otherwise.
 
#15 ·
If you listen to the talk, you will find the explanation for the lessening of the problem.

Fact is that the pesticide companies knew all along that the neonics were causing CCD, so they lowered the pesticide concentration in the seed dressings, consequently CCD is not as prevalent anymore.

http://worcestercountybeekeepers.co...lication-of-honeybee-colony-collapse-disorder

Another interesting detail from this talk: apparently the half-life of imidacloprid in soil is 20 years!
 
#19 ·
Re: Imidacoprid linked to CCD

...even the Harvard press office understood that the symptoms that were induced were not consistent with CCD...which is why the press release contradicts the actual study (and the presentation), and states that some young workers were in the empty hives...there were not, but it makes the symptoms sound more like CCD.

deknow
 
#20 ·
Re: Imidacoprid linked to CCD

"Wait a minute, its all coming back to me now. I am certain it was 1982, I remember because it was the same year that the alien flying saucer crashed in Roswell. I will always believe that somehow those aliens were responsible and that the government was just covering it all up."

I think you are correct Jim. My wife's first cousin's first husband worked with a fellow whose brother was working as a wrecker driver in Rosland in that year. He was called out on a job and sworn to secrecy by the "Men in Black." He had the only wrecker in the area big enough to haul the demobilized space ship to the under ground secret cavern. To this day, he will not demonstrate the super secret hand shake required to enter the cavern. He claims to this day that the aleins brought bees on their space ship, and that they were deeply interested in our domestic bees. The NSA fellow that my wife's knew felt like the aliens civilization had failed due to CCD. My connection said the NSA people could not cipher CCD. It was a meaningless term to them at the time. All these many years later, CCD is still undefined.
 
#21 ·
Re: Imidacoprid linked to CCD

This from Joe Waggle (who does an amazing job of tracking this stuff down):

In accordance with reporting every colony collapse as CCD, we must include
the following die-offs as CCD events. Especially the 1794 instance in
Edinburgh which IMO is highly symptomatic of CCD.

950, 992 and 1443 Ireland In Ireland, there was a “great mortality of
bees” (Flemming G (1871)

1794 Edinburgh, Midlothian
“The following extraordinary instance of the industry of Bees, happened
this season in a bee hive the property of Mr. John Scotland, Merchant,
Dunfermline.
...What is very remarkable, when
the hive was smoked, there were not above 200 bees in it..

1872 Wisconsin Janesville,
“—A gentleman, in Fond du Lac, who usually keeps a great many bees, states
that at least two-thirds of his bees died last winter. He thinks that from
two-thirds to three-quarters of the bees in the county have died this
year.”

1879 Illinois
“Extraordinary Mortality among Bees… One large bee raiser in this State
who had 220 swarms of bees has now only eighteen, and the other who had
over 800 swarms has now not a single healthy hive of bees. It has been
ascertained by correspondence that in New York and the New England States
over 60 per cent, of the bees have died,..”

1885 United States
"The season of 1884-85 stands out in the history of American beekeeping as
one of terrible devastation" (BEEKEEPING (1915) By E.F. Phillips Pg. 343)

1885 Iowa
“…with great unanimity they denounce the honey dew as the cause of the
unexampled and ruinous losses of bees during the past winter. One bee-
keeper loses fifty-one out of fifty-three colonies, and the two left, are
miserably weak. Others have lost ninety-five per cent…”

1904 United States
"During the winter of 1903-04 probably seventy percent of the bees in New
England died." (BEEKEEPING (1915) By E.F. Phillips Pg. 343)

1904 Wisconsin
“Hard on the Bees. Bee keepers report that the present winter has been an
exceptionally hard one on the swarms, and that as a consequence there will
be a great loss to keepers….”

1905 Texas
“…Hard Winter and Too Much Rain Curtails Industry.…. …Phillips, president
of the Nueces Valley Beekeepers’ Association, states … Last winter the
beekeepers suffered a loss, of probably 50 percent of their stock, and the
rains during the spring have been detrimental to the honey flow in the
flowers up to this time.”

1910 United States
"....in 1909-10 the loss was probably fifty per cent in the northeastern
United States." (BEEKEEPING (1915) By E.F. Phillips Pg. 343)

1910 Nebraska
“… The News learns that all, or nearly all, of the bees in this part of
the state were killed by the severe winter. The owners of a large number
of hives on examination find that the bees died In the early part of
winter. This means a shortage in the honey crop. There is now and then a
hive where there are a few live bees and in some instances a hive or two,
out of many, escape, but the slaughter was the worst known in this section
for years.”

1912 United States
"The winter of 1911-12 was also one of heavy mortality, the actual death
of colonies costing the beekeepers in the eastern United States millions
of dollars." (BEEKEEPING (1915) By E.F. Phillips Pg. 343)

1912 Illinois
“The honey crop In central Illinois, will be light this season, due to the
fact that many of the Insects were killed by the severe temperature of
last winter. “

1917 California
“…Winter losses of bees range from 10 to 15 per cent, and in some states
the loss was almost 50 per cent during the winter of 1916-17,…”

1996 New York
“Two ferocious mites are decimating a bee population already weakened by
two straight harsh New York winters….” "It's devastating." Just ask
commercial beekeeper John Earle of Locke, who lost 70 percent of his 900
hives to the mites…”
 
#22 ·
Re: Imidacoprid linked to CCD

Finally! Some answers and some good science. uhhhhhh that would be Lazy Shooter, not sure what Dean is rambling about :D
 
#23 ·
Re: Imidacoprid linked to CCD

...sorry, I was distracted by my conference call...I'm waiting for my orders from the home office (Bayer) on what I should post next. It is top secret, so it is coming via BHC (black helicopter courier)...shhhh, don't tell...i'm going to have an inground pool installed in the shape of a crop duster with the $$$$$).

...does that make more sense?

deknow
 
#24 ·
Re: Imidacoprid linked to CCD

..more from Joe....

Report of the Secretary of Agriculture
By United States Dept. of Agriculture

=====Article Start=====

Agricultural Report
Statistics of Beekeeping

During the past season a disease suddenly appeared in several states,
sweeping away whole apiaries. So quiet were its operations that the bee-
keepers became aware of its existence only by the disappearance of their
bees. The hives were left, in most cases, full of honey, but with no brood
and little pollen; the whole appearance of the hive causing the casual
observer to suppose that the bees had "emigrated;" but close observation
showed that they had died. We give a number of accounts from various
correspondents, principally from the states where this disease first raged.

Jesse R.. Newson, Bartholomew County, Indiana, says: "With an experience
of twenty-five years, I have not seen so disastrous results among bees as
in the present year. We generally feel that all is well with our bees, if
they have succeeded well in laying up a winter supply of food. I have lost
nineteen stands since the first of November; in some of them as many as
forty pounds of honey were left, looking very nice, and tasting as well as
any I ever saw; no sign of moth or any thing wrong that I could see. The
bees seem to die without a cause. The stand twenty years old is yet
living. We find in nearly every stand plenty of food, but "what ails the
bees? What the remedy? If something is not done to stop this fatality,
this pleasing and useful pastime will be taken from us, and our tables
will be robbed of honey."

A. Leslie, Pike County, Indiana, says: "Nearly all our bees have died in
this county, perishing mostly in November, supposed to be for want of bee-
bread."

S. G. Bates, Boone County, says: "The mortality among the bees this winter
cannot be accounted for, since they have plenty of food. Out of twelve
hives I this day took three hundred pounds of honey"; not a young bee to
be found; the comb clear and healthy. My opinion is, that the queen, from
some reason, not having deposited eggs, is the cause of their death."

T. J. Conuett, of Austin, Scott County, Indiana, says: "There is a disease
prevailing to an alarming extent among our bees this fall that is entirely
new, nobody being able to find any cause or remedy. Old and substantial
swarms die, leaving the hive full of honey and bee-bread. Full three-
fourths of the swarms are dead, as far as I have heard from them."

J. N. Webb, Newcastle, Henry County, Kentucky, says : " There were no
swarms last spring, so far as is known. The bees, however, continued
to work and lay up their stores until some time in August, or early in
September, when, to the consternation and utter surprise of the bee-
raiser, they were all found to have died. Many swarms left well-stored
stands of excellent honey, amply sufficient to carry them through the
winter; and what is more strange, comparatively few of the bees were
found dead at the hives. What was the cause of the wholesale destruction
of this useful and interesting insect, dying in the midst of plenty, away
from its hive, we cannot understand. Up to the time when the discovery was
made, no frosts had come, no atmospheric change had taken place, out of
the ordinary course, and in fact nothing to which it may have been
rationally attributed."

T. Hullman, jr., of Terre Haute, Indiana, writes as follows: " In
September last, when the first cold weather set in, my bees began to die.
First, I found in one of my best stands, with all the frames full of
sealed honey, and some honey in boxes, the bees all dead. After that the
bees began to die in all my stands, mostly pure Italians, and some
hybrids. First, about one-third of the bees would be found dead; next, I
would find the queen lying dead before the hive; and in about a week more,
the whole colony would be found dead in and around the hive. Sometimes the
queen would live with a handful of bees. The hives were full of honey,
gathered the latter part of the season ; and the smallest had enough for
the bees to winter upon. In this way I have lost forty stands, and have
now only fifteen skeleton colonies, which I think will also perish before
spring. At first I thought I was the only victim, but I have ascertained
that all the bees in this neighborhood have died, and as far as thirty
miles north and eighteen south. Yesterday I saw a letter from Kentucky,
from a man who thought his bees had stampeded in the same manner as mine,
to the hive of mother-earth. Some colonies had broods others had not. Late
in October all the queens commenced laying again. To some colonies I gave
three queens in about two weeks, and they lost each in turn."

The true cause of the disease has not been discovered. Some attribute it
to the want of pollen; some to poisonous honey; and some to the unusually
hot summer. Whatever may be the cause, the effect has been most
disastrous, throughout these two States.

=====End Article=====


It may appear CCD is making a resurgence. You may perhaps have noticed
the old writing style, or terms used in the Agriculture Report. If you
have, good work, because the article is from 1869, reporting on the
honeybee mortality of 1868.

Is history repeating itself? There are a few similarities, not only with
some of thesymptoms, but more strikingly, in the human reaction.

As the CCD of today has some calling it the AIDS of bees, In the years
following the 1868 bee mortality, that disease has been paralleled to
another disease feared by humans at that time, -Cholera. The bee disease
of 1868 became known as “Bee Cholera”.

In fact, in the years following the 1868 bee mortality, the panic was so
severe with consumers, newspaper reports state that “consumers refuse to
buy what is called Bee Cholera Honey, -where the bees have died of this
disease, consumers don’t like it, and the doctors think it is dangerous to
life.”

In 1869, fear spreads amongst beekeepers the same as with today’s CCD,
which as in both instances, created a need for blame. During the Bee
Cholera die off, articles in the Bee journals quote beekeepers as
saying: “Bee Cholera was not known in the United States until the Italian
bee was introduced”, and the belief that the Italian bee was to blame
endured for years.

In the article below, please find the symptoms associated with the Bee
Cholera,of 1868, so you can make your own diagnosis.

Page 34 Annals of Bee Culture

The Bee Cholera of 1868.

By D. L. Adiar.

During the fall of 1868 and following winter, honey bees died in
great numbers throughout a large portion of the States of Ohio,
Indiana and Kentucky, in a manner not before noticed in diseases of
that insect. All the bees in some large Apiaries died or disappeared, and
over large districts scarcely a colony escaped, and the few that still
survive
are in a diseased and weak condition. From my own observation,
and a correspondence with parties, in different parts of the infected
district, I have ascertained the existence during the fall and winter of
following unusual conditions of the hives.

1. The honey stored by the bees from about the 20th of July was of
a bad quality. When taken from the hives it fermented in a short while,
and a great deal of it fermented in the hives.

2. The honey not only fermented in the cells that were uncapped, but
in those that had been sealed up.

3. The fermentation partially decomposed the wax covers of the cells,
turning them an ashy gray, giving them a bleached or faded appearance,
and bubbles or froth oozed through.

4. The honey was of a peculiar reddish color, and somewhat turbid,
and of a bitter disagreeable taste.

5. In the first stages of the fermentation, the honey was viscous, slimy
or ropy. It afterwards lost its viscous character, and emitted an odor
like rancid butter, which I suppose to be butyric acid, developed perhaps
by the decomposition of the wax. The honey up to this time (1st of
Feby. 1869) still retains some of that odor, and is yet turbid.

6. The bees did not commence dying until the honey in the hives
showed fermentation, about the 20th of August.

7. There was an unusual activity about the diseased hives, the bees
flying in great numbers before and around the hives, and excitedly
running in and out, apparently greatly confused or disturbed.

8. The abdomen of the bees, after death, was considerably swollen, and
filled with an offensive fluid, and some of them are now, after being dead
four months, as soft and pliant, as if they had just died, not having
stiffened or dried up in the least

From these observations I infer that the disease was induced by the
unhealthfulness of the honey.

Best Wishes,
Joe
http://pets.groups.yahoo.com/group/HistoricalHoneybeeArticles/
 
#25 ·
Re: Imidacoprid linked to CCD

Yes, history is repeating itself w/ periodic diebacks of bees. It seems to happen cyclically (sp?).

AIDS = Apiary Inspector Disease Syndrom. This was first stated in an article in The Speedy Bee many years ago. A cpl of decades ago actually, I believe.
 
#28 ·
Re: Imidacoprid linked to CCD

Please give the credit where it is due...to Joe Waggle....he does an incredible job with his historical research and references.

deknow
 
#30 ·
Re: Imidacoprid linked to CCD

You need to Contact Dr. Jeff Pettus and let him fill you in on the collapse that happened in Alabama back in the early 90's. It was the first incident of CCD like symptoms in the USA other than the "disappearing diseases" that happen cyclical from 1972 on back into history. Which brings me to the point of the posting. A lot of colonies that have died from CCD in the past few years are not located near crops sprayed with pesticides. These apiaries are located out in woodlots and pastures. Nor were they moved to crops for pollination purposes. They are located in a closed border state were it takes a compliance agreement to cross the border with bees on comb. So there is more than one culprit as has been suggested in scientific data for the cause of CCD. TED
 
#32 ·
I guess I'm hung up on "accuracy". The reports noted above should be given due credence. Mass bee die-off's have happened for a very long time. We don't even begin to have an idea of all the possible causes.

DarJones
 
#34 ·
If beekeepers would deal w/ the things which kill their bees in an effective manner we could reduce diebacks by a great percentage. I haven't seen a recent AIA report on what colony deaths across the Nation are attributed to by category, but, CCD related causes of colony mortality were a small portion of the 30% winterloss of a cpl of years back. And this years winterloiss seems less amongst folks that I know of and hear of.

So, if people can address problems which can be addressed, starvation, varroa mites, etc., maybe we could just accept that there are some colony mortalities which we can't diagnose and just get on w/ our beekeeping w/out bemoaning a small percentage of loss.
 
#36 ·
Here is an article coauthored by Dennis vanEngelsdorp:
http://www.beeculture.com/content/C...byn M. Underwood and Dennis vanEngelsdorp.pdf
Similar to the historical list that deknow posted. The fact that there have been mass die-offs of honeybee populations in the past does neither prove no disprove any effects of neonicotinoids on honeybees. It is irrelevant to a discussion of neonicotinoids, it can be presented as an argument just as a "the gamblers fallacy" in having some belief in "runs" that are independent of statistics, but it is irrelevant.

Here is a more recent article coauthored by Dennis vanEngelsdorp which demonstrates the effects of imidacloprid (at levels of exposure commonly encountered) on honeybee health.
The finding that individual bees with undetectable levels of the target pesticide, after being reared in a sub-lethal pesticide environment within the colony, had higher Nosema is significant. Interactions between pesticides and pathogens could be a major contributor to increased mortality of honey bee colonies, including colony collapse disorder, and other pollinator declines worldwide.
http://www.springerlink.com/content/p1027164r403288u/fulltext.html
 
#37 ·
The fact that there have been mass die-offs of honeybee populations in the past does neither prove no disprove any effects of neonicotinoids on honeybees.
...and the corollary is that demonstrating that insecticides are toxic to insects says nothing about the cause of dieoffs. This is what we are discussing here...the study in question claims to connect imidacloprid and a series of bee die offs...yet the symptoms of the dieoffs don't match the induced symptoms by feeding imidacloprid. The facts are that die offs (boom and bust) are an integral part of insect populations. The historical record is that there have been dieoffs that resemble what is being reported today since well before neonics were invented.


Here is a more recent article coauthored by Dennis vanEngelsdorp which demonstrates the effects of imidacloprid (at levels of exposure commonly encountered) on honeybee health.
...except that the effect was only observed in caged trials...Jeff Pettis is quoted as saying that they did not see the same effect on free flying colonies. They also did some curious things....like not testing the bees that died in the cages for imidacloprid residue. Bees are not bees when they are in cages.

deknow
 
#47 ·
Land invertebrates are difficult to study the effects of the exposure to contaminants. We have accepted standards for bioassays for aquatic Ceriodaphnia dubia, Pimephales promelas and Mercenaria mercenaria but, bioassays are not a requirement for introducing a new contaminant into the environment.

Terrestrial vertebrate testing we have standards for mice, rats and dogs, but bioassays are rare and are not a requirement. Toxicity testing should also be performed on annelids, insects and birds. There must be a reason why we cannot reach a consensus on an acceptable standard for a bioassay of a land invertebrate.

Oh yeah. . I forgot. It cost industry too much. Let the taxpayers pay for the cleanup years and decades later.

Want to look at history repeating itself? There is an extensive chronology. We don't like to think about those things. We just palm it off someone else to take care of.
Want to look at a resource map of a typical small town in the USA of known groundwater contamination? How about an overlay map of the same town from the CDC of pancreatic cancer cases? What about 300 maps of different towns in the USA?
You can't prove it. All we can do is demonstrate that there are health effects associated with something.
Mathematics itself is only a theory.
 
This is an older thread, you may not receive a response, and could be reviving an old thread. Please consider creating a new thread.
Top