I've been schooled that the best larvae to make into queens are those larvae that are 24 to 36-hours old. As I use my Nicot system, those young larvae are clearly fed royal jelly, but it also seems the royal jelly is fed well into the third day (48 to 72 hours).
If one difference between good queens and poor queens is the age of the larvae that are grafted, and since these larvae are still being fed royal jelly, is the 24 to 36-hour window really that critical? What am I missing here?
Second, with the Nicot system, I can see the larvae clearly as my close-up, uncorrected vision is quite good. When does this larvae begin to show the segmentation of it's body? I keep going back to the idea, expressed somewhat ironically, that if you can see the larvae, they're too big to graft.