If so, these are II queens waking up after receiving their 2nd CO2 treatment. We have a really nice column CO2 "gasser" that can do a bunch of queens at the same time. A really nice gentelman in Kelly's lab made this for us years ago--he was really bored and waiting retirment. Making tools for our II set-up was interesting for him and he made us all kinds of cool stuff. We don't use this that much becasue we're giving virgins their first CO2 treatment the day before they are insmeinated, usually out in the field.