I've got a hive (its first year) that has the side of one frame virtually covered in a second layer of comb. I had forgotten to put the 9th frame into the brood box, so the bees had a little more room than I had intended.

Honey is beginning to flow copiously (I'm in Minnesota), and the bees are very active.

I'm a little afraid to take that second layer off and toss it, because the queen might be there. (The queen is unmarked, since the hive is in its first year and the packaged queen is unmarked.)

Should I leave that second layer in place, or take it off?