I had a nice plump laying queen, I gently placed her into my clear plastic queen marking tube, then pushed the foam insert plunger until she was gently trapped against the plastic mesh in a way that would allow me to place a dab of paint on her thorax. I've done this more than a hundred times now, without a single incident. This time when I released the plunger and the pressure holding the queen immobile was gone, the queen just lay their twitching, her abdomen was spasming and an egg was ejected from the tip of her abdomen. The abdominal twitching continued and every other part of her body appeared paralyzed. Her legs were not moving and neither was her head. I was afraid that she was a goner.
I don't like to give up easily, so I placed her on a frame of her brood and nurse bees. They quickly went to her aid, I saw one look like it was feeding her, and they all were giving her a great deal of attention. I carefully placed that comb against the adjacent comb (hoping that would help keep the queen from falling from the comb), and carefully closed the hive.
I checked them the next morning (that was this morning) and discovered that she had fully recovered and was busy laying eggs. Time will tell if she is disabled in a less than obvious manner (such as being rendered infertile), or having her vitality or life shortened.