Here's one option you can try...
Get a metal queen excluder and nail/screw/staple it solidly to the bottom of a beebox. Put this on top of another empty bee box that has a top lid nailed/screwed/stapled to the BOTTOM of the box.
Now you have a queen and drone 'sifter' if you will. You can now shake or brush all frames from your hive into this top box and smoke the bees down into the bottom box, and shake the top box aggitatinly to sift the bees down thru the Qexcluder. If you have a qeeen, she won't go thru the excluder thereby you found her. Then take the box they were sifted into and turn upside down back onto your hive and they'll flow down into it.
20+ years, raise my own queens, feed when needed. I treat, but have not perfected varroa management yet.