I have a lang that has been queenless for 22 days
during that time they have refused two lovely Russian Ladies.

There is no brood of any sort (from egg to capped larvae)...

Also I have newly started TBH with 5 frames almost full. Is there a chance for survival of the queenless hive if I put them in an extra TBH and give them one of the frames from the new TBH with brood and eggs in it ?

I would hate to loose both hives by putting too much stress on the nuc in the TBH....