I've got a nuc started to care for the queen I ordered. I've made three attempts to find the virgin, and failed. She should be laying within the week (estimating conservatively), and then I'll open up the hive sans smoke and see if I can run her down and, err, offer her a nice alcohol treatment. That hive has plenty of bees, so I might pull two frames and see how she does, but I want a well mated queen. I'm also reluctant to weaken my decent hive for the purposes of expirementing.