View Full Version : Where to get 100 microliter tubes
magnet-man
12-28-2008, 11:10 AM
I am about out of 100 microliter tubes. I am getting ready to fabricate a tip maker and need a supply of tubes. Does anyone know where to get the same type of tubes that Sue uses?:s
I am about out of 100 microliter tubes. I am getting ready to fabricate a tip maker and need a supply of tubes. Does anyone know where to get the same type of tubes that Sue uses?:s
The following site mentions the kind needed and I found a lot of glass companies that offer glass capillaries via Google. You can take some callipers to what you've got already for measurements (OD/length) and order from Fisher Scientific. I like the idea of using an ID of 1.128 mm so each mm of length equals 1ul of volume.
http://www.dave-cushman.net/bee/makingtips.html
I'm still waiting to give II a try so I can't weigh in with any experience.
magnet-man
12-28-2008, 05:54 PM
Sue used blue banded and have the 100 ul marked. I have no problem finding the 100 ul but can't seem to find the marked ones.
Sue used blue banded and have the 100 ul marked. I have no problem finding the 100 ul but can't seem to find the marked ones.
Sue is really nice so maybe you should drop her a line and ask.
Any of the lab supply companies sell the glass capillary tubes, Fisher, VWR... They often want you to establish an account and place a minimum order, so try searching the internet for other deals. Sometimes they are listed on Ebay.
I do sell small quantities of the 100 microliter tubes on my website.
On a side note, tips are normally made from 50 microliter capillary tubes.
Good Luck,
Joe
magnet-man
12-28-2008, 07:02 PM
Joe, you are right. I didn't look that closely at my tips and they are green banded. Silly me for not looking closer.
Joe did you make your own tube puller or do you have a Schley tube puller like Sue's?
I actually have an old vertical pipette puller. I do not remember the company that made it as it is built like a tank and almost over-kill for pulling such small pipettes. The vertical style pullers seem to produce a much more uniform taper than the horizontal pullers around today.
Unfortunately, I do not think Schley makes/sells his pullers any more. It is a really nice and simple design.
Joe
magnet-man
12-28-2008, 08:17 PM
You are right, Schley's design was elegant in its simplicity.
Aspera
12-29-2008, 11:52 PM
Search "electrophysiology" or "patch-clamping" supplies. If that doesn't work, you might be able to get one of the larger breeders to divert some tubes from BD or VWR biological supplies.